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The European Arabidopsis Stock Centre

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NASC ID: N634960

Name: SALK_134960

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g80750 F23A5.1 0 Intron F23A5.1 0 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_134960.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G11690.1 and AT1G11700.1=P355 (at chr 1 pos 3943416 (W/3943358-3943416) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 63, R 69, C 69 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357269.
SALKseq_134960.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G17410.1 (at chr 1 pos 5969879 (W/5969801-5969879) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 63, R 69, C 69 N 66. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360623.

Donation Date: 2003-03-11

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_134960

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664073 (SALK_134960C).

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.