Home | About NASC | Address/Staff
Links | Ask a question | Help

NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N2106367

Name: SAND Seed Set

Description: Each SWELL line construct was transformed into C58 GV3101 Agrobacterium strain and selected on YEB media (5g/L beef extract; 1g/L yeast extract; 5g/L peptone; 5g/L sucrose; 15g/L bactoagar; pH 7.2) supplemented with antibiotics (Spectynomicin, Gentamycin). After two days of growth at 28°C, bacteria were collected using a single-use cell scraper, re-suspended in about 200mL of transformation buffer (10mM MgCl2; 5% sucrose; 0.25% silweet) and Col0 plants were transformed by dipping. Primary transformants (T1) were selected in vitro on glufosinate. For all constructs, around 20 independent T1 lines were isolated and six representative mono-insertion lines were selected in T2. Independent lines homozygous for the transgene were selected in T3. Some of the lines were produced later during the selection process and are distributed as segregating T2s.

Donation Date: 2015-10-29

Donated by:  Ecole Normale Supérieure de Lyon Yvon Jaillais
Centre National de la Recherche Scientifique (CNRS) Gregory Vert

Stock type: set (View set contents)

Material type: seed

Comment: Donor names: Yvon Jaillais, Gregory Vert.

Status Price (£)
Available £58.00

Germplasm Info

Seed type: T-DNA Insertion

Mutagen: agrobacterium tumefaciens

Background: Col

References

Mar Marquès-Bueno, M. et al. 2016. A versatile Multisite Gateway-compatible promoter and transgenic line collection for cell-type specific functional genomics in Arabidopsis. The Plant Journal 85(2):320-33. PubMed ID: 26662936.