Baker Ds Cre-lox
Donated by
- Barbara Baker The Plant Gene Expression Center
- Cathy Corr The Plant Gene Expression Center
- Brian Osborne The Plant Gene Expression Center
- Joshua Levin Division of Biology, California Institute of Technology (Caltech)
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Description
Baker Ds Cre-lox
transformed with T-DNA lox-Dslox comprised of: CaMV 35S - lox (pairs of lox sites recombine site-specifically in presence of Cre transposase) - surA,B (chlorsulfuron resistance) interrupted by a Ds element, so that the exisiting line is chlorsulfuron susceptible; the Ds element contains CaMV 35S - bar (PptR gene, conferring phosphonothricin resistance), a lox site adjacent to a promoterless AAC (encoding gentamycin-3-N-acetyltransferase, conferring gentamycin resistance); the Dslox construct is in R orientation; supF from yeast; Nos-NptII (KanR gene conferring kanamycin resistance); combined phosphonothricin, chlorsulfuron selection after crosses to Ac line (CS8110) confirms transposition events; gentamycin selection after crosses to Cre line (CS8111) confirms lox-lox recombination with potential chromosomal rearrangements
other information
- clone name: lox-Dslox
- construct type: cre-lox_recombination
References
- Osborne B I, Wirtz U, Baker B, . A system for insertional mutagenesis and chromosomal rearrangement using the Ds transposon and Cre-lox. 1995 04; 7(4) . Cited in PubMed; PMID 7742862